Lo studio espone dei topi all'azione dei campi elettromagnetici pulsati, questi manifestano deficit cognitivi e della memoria con evidenze di accumulo di proteina beta amiloide a livello neuronale ( tipico per malattia di Alzheimer )
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Arch Med Res. 2013 Apr;44(3):178-84. doi:
10.1016/j.arcmed.2013.03.005. Epub 2013 Mar 19.
ELECTROMAGNETIC PULSE EXPOSURE INDUCES OVER-EXPRESSION
OF BETA AMYLOID PROTEIN IN RATS.
Author information
1Department of Radiation Medicine, Faculty of
Preventive Medicine, Fourth Military Medical University, Xi'an, Shaanxi, China.
ABSTRACT
BACKGROUND AND AIMS: With the developing and widely used electromagnetic
field (EMF) technology, more and more studies are focusing on the relationship
between EMF and Alzheimer's disease (AD). Electromagnetic pulse (EMP) is one
type of widely used EMF. This study aimed to clarify whether EMP exposure could
induce cognitive and memory impairment, thus finding a possible relationship
between EMP and AD.
METHODS: Forty healthy male Sprague Dawley rats were randomly
divided into four groups. Animals, respectively, received 100, 1000, and 10,000
pulses EMP (field strength 50 kV/m, repetition rate 100 Hz) exposure and sham
exposure when 2 months old. Monthly Morris water maze (MWM) was used to test the
changes of cognitive and memory ability. Superoxide dismutase (SOD) activity
and glutathione (GSH) content were used as oxidative stress indexes.
Expressions of some types of Alzheimer's disease-related proteins were also
detected.
RESULTS: After exposure, EMP exposure caused clear cognitive
and memory impairment compared with sham exposure group (p <0.05).
Determination of oxidation indexes showed decreased SOD activity and GSH
content in exposure groups compared with sham group. Immunohistochemical (IHC) staining showed
increased beta amyloid protein (Aβ) in EMP exposure
groups compared with sham group. Western blot experiments showed increased
expressions of Aβ oligomer and beta amyloid protein precursor (APP) in
EMP exposure groups. Increased expression of microtubule-associated protein
1 light chain 3-II (LC3-II) was also found.
CONCLUSIONS: The present results
showed that EMP exposure can cause long-term impairment in impaired cognition
and memory of rats, resulting in AD-like symptoms. This may be induced by
enhancing oxidative stress and is related to autophagy dysfunction.
