In questa pubblicazione si evidenziano ulteriori meccanismi
d'azione indotti dalle basse frequenze (ELF-EMF) a livello cellulare. Queste
nuove conoscenze potrebbero essere importanti per stabilire un quadro all'interno del quale comprendere i possibili effetti dei campi ELF
sulla salute.
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- 1Department
of Medicine and Aging Science, University 'G. d'Annunzio' of Chieti-Pescara,
Via dei Vestini, Chieti, Italy.
- 2King Fahd
Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
- 3Department
of Bioengineering, Integral University, Kursi Road, Lucknow, UP, India.
- 4Department
of Experimental and Clinical Sciences, University "G.d'Annunzio"
Chieti-Pescara, Chieti, Italy. Electronic address: mreale@unich.it.
ABSTRACT
AIMS:
Extremely low frequency electromagnetic fields (ELF-EMFs) are
widely employed in electrical appliances and different equipment such as
television sets, mobile phones, computers and microwaves. The molecular
mechanism through which ELF-EMFs can influence cellular behavior is still
unclear. A hypothesis is that ELF-EMFs could interfere with chemical reactions
involving free radical production. Under physiologic conditions, cells maintain
redox balance through production of ROS/RNS and antioxidant molecules. The
altered balance between ROS generation and elimination plays a critical role in
a variety of pathologic conditions including neurodegenerative diseases, aging
and cancer. Actually, there is a disagreement as to whether there is a causal
or coincidental relationship between ELF-EMF exposure and leukemia development.
Increased ROS levels have been observed in several hematopoietic malignancies
including acute and chronic myeloid leukemias.
MAIN METHODS:
In our study, the effect of ELF-EMF exposure on catalase,
cytochrome P450 and inducible nitric oxide synthase activity and their
expression by Western blot analysis in myelogenous leukemia cell line K562 was
evaluated.
KEY FINDINGS:
A significant modulation of iNOS, CAT and Cyt P450 protein
expression was recorded as a result of ELF-EMF exposure in both phorbol
12-myristate 13-acetate (PMA)-stimulated and non-stimulated cell lines.
Modulation in kinetic parameters of CAT, CYP-450 and iNOS enzymes in response
to ELF-EMF indicates an interaction between the ELF-EMF and the enzymological
system.
SIGNIFICANCE:
These new insights might be important in establishing a
mechanistic framework at the molecular level within which the possible effects
of ELF-EMF on health can be understood.
FONTE :
