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giovedì 29 gennaio 2015

EFFECTS OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELD (ELF-EMF) ON CATALASE, CYTOCHROME P450 AND NITRIC OXIDE SYNTHASE IN ERYTHRO-LEUKEMIC CELLS.

In questa pubblicazione si evidenziano ulteriori meccanismi d'azione indotti dalle basse frequenze (ELF-EMF)  a livello cellulare. Queste nuove conoscenze potrebbero essere importanti per stabilire un quadro all'interno del quale comprendere i possibili effetti dei campi ELF sulla salute.
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.Patruno A1, Tabrez S2, Pesce M1, Shakil S3, Kamal MA2, Reale M4.

Author information

  • 1Department of Medicine and Aging Science, University 'G. d'Annunzio' of Chieti-Pescara, Via dei Vestini, Chieti, Italy.
  • 2King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • 3Department of Bioengineering, Integral University, Kursi Road, Lucknow, UP, India.
  • 4Department of Experimental and Clinical Sciences, University "G.d'Annunzio" Chieti-Pescara, Chieti, Italy. Electronic address: mreale@unich.it.

ABSTRACT

AIMS:

Extremely low frequency electromagnetic fields (ELF-EMFs) are widely employed in electrical appliances and different equipment such as television sets, mobile phones, computers and microwaves. The molecular mechanism through which ELF-EMFs can influence cellular behavior is still unclear. A hypothesis is that ELF-EMFs could interfere with chemical reactions involving free radical production. Under physiologic conditions, cells maintain redox balance through production of ROS/RNS and antioxidant molecules. The altered balance between ROS generation and elimination plays a critical role in a variety of pathologic conditions including neurodegenerative diseases, aging and cancer. Actually, there is a disagreement as to whether there is a causal or coincidental relationship between ELF-EMF exposure and leukemia development. Increased ROS levels have been observed in several hematopoietic malignancies including acute and chronic myeloid leukemias.

MAIN METHODS:

In our study, the effect of ELF-EMF exposure on catalase, cytochrome P450 and inducible nitric oxide synthase activity and their expression by Western blot analysis in myelogenous leukemia cell line K562 was evaluated.

KEY FINDINGS:

A significant modulation of iNOS, CAT and Cyt P450 protein expression was recorded as a result of ELF-EMF exposure in both phorbol 12-myristate 13-acetate (PMA)-stimulated and non-stimulated cell lines. Modulation in kinetic parameters of CAT, CYP-450 and iNOS enzymes in response to ELF-EMF indicates an interaction between the ELF-EMF and the enzymological system.

SIGNIFICANCE:

These new insights might be important in establishing a mechanistic framework at the molecular level within which the possible effects of ELF-EMF on health can be understood.

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